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12 of 2 x 5ml Syringe Pairs of US-EPA-1002/03 (no EDTA) (Gamma Irradiated)
AlgaBoost EPA-1002/1003 no EDTA is a 400x concentrate of the medium used for Toxicity Testing. It is identical to AlgaBoost EPA-1002/1003, except for the absence of EDTA.
Application : medium of choice for heavy metal toxicity tests; the exclusion of EDTA from this formulation allows more sensitive detection of heavy metals.
Makes: When diluted in water according to instructions, it makes the following media:
1. EPA Test Method 1002.0 (but EDTA free) (Daphnid Ceriodaphnia dubia, Survival and Reproduction Test).
2. EPA Test Method 1003.0 (but EDTA free) (Green Alga, Selenastrum capricornutum, Growth Test).
To Make:
2 litres distilled water
+ 1 x 5ml Syringe Part A
+ 1 x 5ml Syringe Part B
2 litres USEPA-1002/1003 medium (EDTA-free)
Nutrient Balance: When added to your seawater + freshwater mix (seawater : freshwater = 4 : 1.1 ) according to the accompanying
instructions, the combined parts of this formulation will provide the following nutrient levels:
| Nutrient | Final conc per litre (g/l) |
|---|---|
| NaNO3 | 25.5 mg |
| NaHCO3 | 15 mg |
| MgSO4.7H2O | 14.7 mg |
| MgCl2.6H2O | 12.2 mg |
| CaCl2.2H2O | 4.41mg |
| K2HPO4 | 1.04 mg |
| MnCl2.4H2O | 0.416 mg |
| H3BO3 | 0.185 mg |
| FeCl3.6H2O | 0.160 mg |
| CoCl2.6H2O | 0.00143 mg |
| ZnCl2 | 0.00327 mg |
| Na2SeO4 | 0.00239 mg |
| CuCl2.2H2O | 0.000012 mg |
| Na2MoO4.2H20 | 0.00726 mg |
Nutrient Balance: When added to freshwater according to the accompanying instructions, this formulation will provide the following nutrient levels:
How is the product supplied ? - As a 2-part mix, in packs containing 12 kits (enough to make 12 x 2 litres of EPA 1002/1003 medium). Each part of the 2-part mix is sterile and is provided in a convenient 5ml syringe; the two syringes are provided in a sealed sterile pack.
To Make the Medium: - Open a 2-syringe kit. Then add the the contents of one 5ml syringe to 2 litres of pure water, mix thoroughly, then add contents of the other 5 ml syringe and mix (instructions accompany the product). Aerate the mixture, adjust pH if necessary (recommended for EPA method 1003.0) and filter sterilise.
NOTE: EPA-1002/1003 is sterile; the final sterilisation step (described above) is necessary only if diluent (water) is not sterile and/or if sterile technique has not been used while preparing the final medium.
Reference (EDTA-version): "Short-term methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms." Eds: P.A. Lewis, D.J. Klenni, J.M Lazorchak, T.J. Norberg-King, W.H. Peltier, M.A. Heber 1994.
Availability: 'EPA-1002/1003 no EDTA' is presently available only in the syringes. If you are interested in purchasing larger amounts, please enquire with us.
$120.00
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