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1 x 10 Litre Drum Harrisons 1000x (Gamma Irradiated)

1 x 10 Litre Drum Harrisons 1000x (Gamma Irradiated)

AlgaBoost (1000x) Harrisons is a sterile 1000x concentrate of Harrisons# medium for culture of marine microalgae.
 
Makes:    
9,900 litres x sea water
+    10 litres x 1000x Part A
+    10 ml x 1,000,000x Part B
=    10,000 litres Harrisons
 
Nutrient Balance: When added to seawater according to the accompanying instructions, the combined parts of this formulation will provide the following nutrient levels:
                      
To Make Harrisons modified medium: Pour 1 litre from the 10 litre drum (Part A) into a sterile culture bag. Add 1000 litres of sterile seawater and mix thoroughly. Add 1 ml of Part B (contained in the 10 ml syringe). Now you've got 1000 litres of Harrisons modified medium.
 
Can I use Harrisons 1000x with diatoms ? The exclusion of silicate from our Harrisons 1000x concentrate means it is unsuitable for use with diatoms (unless you add silicate to your seawater yourself).
 
Formulation:  Harrisons (1000x) is formulated as described by Harrison et al (1980) but is more concentrated, uses sodium hydrogen phosphate in place of B-glycerophosphate and excludes silicate.
 
#Ref:    Harrison, P.J., Waters, R.E. & Taylor, F.J.R. "A broad spectrum artificial seawater medium for coastal and open ocean phytoplankton": J. Phycol. 16: 28-35, 1980
 
Product Conditioning: contents of the 10 litre drum and the 10 ml syringe, are 0.2 micron filtered.
 
Nutrient Final conc per litre
 
Nutrient Final conc per litre
NO3- 34 mg
NH4+ 0.2 mg
Na+ 15 mg
PO43- 2.44 mg
BO33-   3.6 mg
Fe3+    0.033 mg
Fe2+ 0.333 mg
Mn2+    0.13 mg
Co2+ 0.0033 mg
Zn2+    0.017 mg
SO42- 1.4 mg
Cl-    0.49 mg
Thiamine HCl    0.1 mg
Vit B12    0.0005 mg
Biotin    0.0005 mg
              
 
To Make Walnes: Pour 1 litre from the 10 litre drum (Part A) into a sterile culture bag. Add 1000 litres of sterile freshwater and mix thoroughly.  Add 1 ml from the 10 ml syringe (Part B) and mix thoroughly.  Now you've got 1000 litres of silicate-free Walnes. 
 
Can I use this with diatoms ? The exclusion of silicate from our Walnes 1000x concentrate means it is unsuitable for use with diatoms (unless you add silicate to your seawater yourself). 
 
Formulation: With the exception of silicate, our formulation of Walnes makes the same medium described by the CCAP.
 
Perfect for carboy-scale & bag-scale microalgae culture.
 
Product Conditioning: contents of the 10 litre drum and the 10 ml syringe, are 0.2 micron filtered.

1 x 10 Litre Drum Harrisons 1000x (Gamma Irradiated)
Price
$195.00
Stock 0
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