
AlgaBoost (1000x) Harrisons is a sterile 1000x concentrate of Harrisons# medium for culture of marine microalgae.
Makes:
9,900 litres x sea water
+ 10 litres x 1000x Part A
+ 10 ml x 1,000,000x Part B
= 10,000 litres Harrisons
Nutrient Balance: When added to seawater according to the accompanying instructions, the combined parts of this formulation will provide the following nutrient levels:
To Make Harrisons modified medium: Pour 1 litre from the 10 litre drum (Part A) into a sterile culture bag. Add 1000 litres of sterile seawater and mix thoroughly. Add 1 ml of Part B (contained in the 10 ml syringe). Now you've got 1000 litres of Harrisons modified medium.
Can I use Harrisons 1000x with diatoms ? The exclusion of silicate from our Harrisons 1000x concentrate means it is unsuitable for use with diatoms (unless you add silicate to your seawater yourself).
Formulation: Harrisons (1000x) is formulated as described by Harrison et al (1980) but is more concentrated, uses sodium hydrogen phosphate in place of B-glycerophosphate and excludes silicate.
#Ref: Harrison, P.J., Waters, R.E. & Taylor, F.J.R. "A broad spectrum artificial seawater medium for coastal and open ocean phytoplankton": J. Phycol. 16: 28-35, 1980
Product Conditioning: contents of the 10 litre drum and the 10 ml syringe, are 0.2 micron filtered.
Nutrient Final conc per litre
Nutrient |
Final conc per litre |
NO3- |
34 mg |
NH4+ |
0.2 mg |
Na+ |
15 mg |
PO43- |
2.44 mg |
BO33- |
3.6 mg |
Fe3+ |
0.033 mg |
Fe2+ |
0.333 mg |
Mn2+ |
0.13 mg |
Co2+ |
0.0033 mg |
Zn2+ |
0.017 mg |
SO42- |
1.4 mg |
Cl- |
0.49 mg |
Thiamine HCl |
0.1 mg |
Vit B12 |
0.0005 mg |
Biotin |
0.0005 mg |
To Make Walnes: Pour 1 litre from the 10 litre drum (Part A) into a sterile culture bag. Add 1000 litres of sterile freshwater and mix thoroughly. Add 1 ml from the 10 ml syringe (Part B) and mix thoroughly. Now you've got 1000 litres of silicate-free Walnes.
Can I use this with diatoms ? The exclusion of silicate from our Walnes 1000x concentrate means it is unsuitable for use with diatoms (unless you add silicate to your seawater yourself).
Formulation: With the exception of silicate, our formulation of Walnes makes the same medium described by the CCAP.
Perfect for carboy-scale & bag-scale microalgae culture.
Product Conditioning: contents of the 10 litre drum and the 10 ml syringe, are 0.2 micron filtered.